Kinetic Analysis of δ Ribozyme Cleavage
نویسندگان
چکیده
منابع مشابه
Specificity of hammerhead ribozyme cleavage.
To be effective in gene inactivation, the hammerhead ribozyme must cleave a complementary RNA target without deleterious effects from cleaving non-target RNAs that contain mismatches and shorter stretches of complementarity. The specificity of hammerhead cleavage was evaluated using HH16, a well-characterized ribozyme designed to cleave a target of 17 residues. Under standard reaction condition...
متن کاملSubstrate Specificity of d Ribozyme Cleavage
The specificity of d ribozyme cleavage was investigated using a trans-acting antigenomic d ribozyme. Under single turnover conditions, the wild type ribozyme cleaved the 11-mer ribonucleotide substrate with a rate constant of 0.34 min, an apparent Km of 17.9 nM and an apparent second-order rate constant of 1.89 3 10 min M. The substrate specificity of the d ribozyme was thoroughly investigated ...
متن کاملIntracellular RNA cleavage by the hairpin ribozyme.
Studies involving ribozyme-directed inactivation of targeted RNA molecules have met with mixed success, making clear the importance of methods to measure and optimize ribozyme activity within cells. The interpretation of biochemical assays for determining ribozyme activity in the cellular environment have been complicated by recent results indicating that hammerhead and hairpin ribozymes can cl...
متن کاملLigand requirements for glmS ribozyme self-cleavage.
Natural RNA catalysts (ribozymes) perform essential reactions in biological RNA processing and protein synthesis, whereby catalysis is intrinsic to RNA structure alone or in combination with metal ion cofactors. The recently discovered glmS ribozyme is unique in that it functions as a glucosamine-6-phosphate (GlcN6P)-dependent catalyst believed to enable "riboswitch" regulation of amino-sugar b...
متن کاملThe Structural Basis of Hammerhead Ribozyme Self-Cleavage
We have captured an 8.7 A conformational change that takes place in the cleavage site of the hammerhead ribozyme during self-cleavage, using X-ray crystallography combined with physical and chemical trapping techniques. This rearrangement brings the hammerhead ribozyme from the ground state into a conformation that is poised to form the transition state geometry required for hammerhead RNA self...
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ژورنال
عنوان ژورنال: Biochemistry
سال: 1998
ISSN: 0006-2960,1520-4995
DOI: 10.1021/bi9809775